Why didnt methanol boil at 90 degree celcius ?

i extracting the phytochemicals from the grounded root samples using methanol as solvent by soxhlet apparatus. i used oil bath to heat the methanol since my heating mantle wasn't available at the...

03 April 2019 2,451 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

01 February 2019 7,351 6 View

Is it necessary to dissolve the methanolic extract in DMSO before checking the antifungal activty of any plant extract?

so, basically i had to do an anti-fungal assay using a plant extract which was extracted using methanol. then i dissolved the extract in methanol to bring 200mg/ml working concentration. I made...

01 February 2019 5,582 5 View

Can somebody please suggest me with an article to assess the antagonistic activity of a fungi on another fungi ?

i am currently looking for some protocols to be followed to assess the antifungal activity of a new fungus against a pathogenic fungi. i have no idea what fungi it is. so, i dont know what type of...

01 February 2019 3,163 17 View

What does the superscripted alphabet in the data table represent in some research articles ?

recently i encountered some research papers where they have super-scripted some alphabets on top of the values. can somebody please explain me what they are trying to infer. i have attached the...

01 February 2019 2,277 0 View

Can someone help me with GC-MS results analysis?

i have recently done a GCMS analysis for the methanolic extracts of plant samples. i am not sure how to interpret the results. can someone help me with any protocol or procedure on how to begin...

01 February 2019 9,491 8 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

11 December 2018 1,622 6 View

How to grow both bacteria and a fungi together in a single media ?

Basically, i am trying to find the antagonistic activity of the bacteria and a pathogenic fungi. what could be the possible way to culture them if the incubation temp is 37 degree celsius for the...

09 October 2018 1,059 2 View

Does nitrocellulose membrane that is used for the southern blotting technique have more binding capacity than the Silica matrix of Dna purification ?

the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does...

07 August 2018 804 4 View

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02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View