the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does during dna purification.
I would expect the nitrocellulose to bind less dna because it has a small surface area and dna binds to the surface of nylon and nitrocellulose. Silica matrix is particulate and the tiny spheres will have a large surface area so should bind more dna
Hello Paul Rutland,
Thank you for your answer sir, what do you think about immobilizing silica on nitrocellulose membrane? how efficient do you think it would be?
I do not know any way of layering silica in its dioxide form ( some organosilanes are sprayable but these are different chemically) but my thoughts are the same that silica particles have a large surface area (4 pi r squared) so increases faster than the area of nitrocellulose it covers so silica alone is already very efficient at absorption and also insoluble so can be easily separated from reagent solutions either by centrifugation, filtration or just washing so I do not see the advantage in binding it to an inert matrix. Another disadvantage of binding to cellulose is that the cellulose itself binds dna but releases it under different conditions from silica which might be confusing in some circumstances
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