Why didnt methanol boil at 90 degree celcius ?

i extracting the phytochemicals from the grounded root samples using methanol as solvent by soxhlet apparatus. i used oil bath to heat the methanol since my heating mantle wasn't available at the...

03 April 2019 2,451 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

01 February 2019 7,351 6 View

Is it necessary to dissolve the methanolic extract in DMSO before checking the antifungal activty of any plant extract?

so, basically i had to do an anti-fungal assay using a plant extract which was extracted using methanol. then i dissolved the extract in methanol to bring 200mg/ml working concentration. I made...

01 February 2019 5,582 5 View

Can somebody please suggest me with an article to assess the antagonistic activity of a fungi on another fungi ?

i am currently looking for some protocols to be followed to assess the antifungal activity of a new fungus against a pathogenic fungi. i have no idea what fungi it is. so, i dont know what type of...

01 February 2019 3,163 17 View

Is it ok to use McFarland turbidity standards for MIC determination of a plant extract against fungi ?

I have prepared a fungal suspension by scrapping out the fully grown fungal culture from the agar plate and homogenised them. i refered some of the literatures where they have used McFarland...

01 February 2019 3,762 3 View

Can someone help me with GC-MS results analysis?

i have recently done a GCMS analysis for the methanolic extracts of plant samples. i am not sure how to interpret the results. can someone help me with any protocol or procedure on how to begin...

01 February 2019 9,491 8 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

11 December 2018 1,622 6 View

How to grow both bacteria and a fungi together in a single media ?

Basically, i am trying to find the antagonistic activity of the bacteria and a pathogenic fungi. what could be the possible way to culture them if the incubation temp is 37 degree celsius for the...

09 October 2018 1,059 2 View

Does nitrocellulose membrane that is used for the southern blotting technique have more binding capacity than the Silica matrix of Dna purification ?

the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does...

07 August 2018 804 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View