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I transformed the plasmid that inserted the gene into E. coli Bl21, then clony pcr appear two bands and plasmid don't have band?
09 August 2023 879 2 View
Hello everyone, I recently had my MS machine cleaned since then i am facing an issue regarding implementation of my method for ascorbic acid using Sequant ZIC HILIC-MS/MS. I see a break in my...
05 August 2023 8,521 1 View
Hello all, In CV QKD, in general, there is two noise sources in the system. the shot-noise, which is the fundamental noise of the signal and arises from quantization of the electromagnetic...
03 August 2023 1,969 5 View
suppose, 1microM of analyte is added into the water sample and then used for testing. Then how to calculate the concentration of analyte found in the final solution?
29 July 2023 1,739 0 View
Hi! Could someone help me to solve this problem? I will coat the 6-well plate with anti-CD3 first. Then, add anti-CD28 into T cell culture and remove T cell culture into 6-well plate. However,...
24 July 2023 944 1 View
I isolated and differentiated bone marrow-derived macrophages from mice. After differentiation I frozen them and kept them in liquid nitrogen. Then, I thaw them and let them recover for 3-5 days...
24 July 2023 9,638 4 View
Hi, I want to extract lipid rafts by using an Optiprep gradient in order to analyze some receptors. After ultracentrifugation and recovery of the fractions, I used TCA (trichloroacetic acid) to...
19 July 2023 280 0 View
I am planning to perform intracellular Ca2+ mobilization assays on 96-well plates using EndoC-βH1 cells. Specifically, I am interested in measuring β-cell responses to glucose and possibly other...
17 July 2023 3,854 0 View
Material or any composite that have least friction between its sirface and air , which decrease s the boundary layer and also delay boundary layer separation..
16 July 2023 8,768 1 View
Dear all I have modeled Viscous damper in ETABS using link but when I perform TH analysis and view the display cumulative response, the energy in VD remains zero. I may me making some mistakes in...
15 July 2023 9,450 0 View
The cell line I used in my experiment is a self-cultured porpoise fibroblast cell line. On the first day, I seeded cells on a 96-well plate and the cell density was 5 x 10^4 cells/mL. The day...
14 July 2023 6,751 5 View
"primers at a concentration of 10 µmol/L" "primers at a concentration of 10 mmol/L" "primers at a concentration of 0.5 μmol/l" I found this concentration from three different article. How...
12 July 2023 1,510 7 View
Hello, Does anyone have information about the ploidy of HT22 mouse hippocampal? I couldn't find anything about in the literature. Thanks!
12 July 2023 1,084 2 View
Why does the laser for optogenetic stimulation need to be shuttered on and off rapidly? Can i use a constant laser light but at low power?
12 July 2023 2,549 3 View
I tried to record electrophysiological activities (eg: action potential) of neurons from cortical organoids at 100-day-olds using whole-cell patch clamp. I was able to get the giga seal easily....
04 July 2023 1,077 5 View
As we know at the end of welding near the plate edges, the temperature rises too much i.e. the maximum temperature in the arc region is higher than all other points. Now, I am looking for a...
03 July 2023 3,383 2 View
I am trying to evaluate stress levels in different developmental stages of rats. In order to do so I need to measure corticosterone in their fecal, urine, or blood samples. Since we are short on...
03 July 2023 3,694 4 View
If using an electric heating plate for digestion
29 June 2023 9,841 1 View
I want to perform MTT assay for BJ-5ta and HaCat cell lines. I want to know before performing the actual experiment how many passages should be done of the cell lines?
27 June 2023 5,387 3 View
Is it plausible that cells exposed to an oxidative stressor would display increased metabolism (as measured through MTT assays)?
26 June 2023 6,989 2 View
Hello! I really hope anyone can help me out. For my master thesis I am comparing cancer cell clones and their reaction to certain targeted inhibitors. Now I would like to perform a two way ANOVA...
24 June 2023 7,229 1 View
Hello, I'd like to include some background for the problem I'm having. I'm working with Candida glabrata isolates in 96 well plates. My plate conditions are: 1*10^6 cells/mL, 5%CO2, 24h...
23 June 2023 8,403 1 View
Hello, I have been trying to linearize a vector through PCR in prep for cloning for several weeks now. I am using Inverse PCR as opposed to enzyme restriction digestion. However, every time I have...
21 June 2023 6,657 1 View
I am working with iPSCs and at a certain point when dividing them, they differentiate into neurons for no apparent reason since they are treated following the same division protocol. Could someone...
21 June 2023 2,479 1 View