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Questions related to Plating
Is it necessary that all element's pattern of an adaptive array antenna (null-steering) have exact similar pattern? If it is, What is the consequence of different element pattern in an adaptive array?
09 June 2024 5,171 2 View
I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...
08 June 2024 166 2 View
What is the reason or importance of diluting an enzyme before an experiment? I'm writing a report on enzyme activity and characterisation and i need help with understanding this: we were told to...
07 June 2024 8,688 2 View
I am working with microglia SIMA9 and I will perform a qPCR. If you have already worked with them, can you suggest me one?
06 June 2024 4,905 3 View
Hi everyone, I am currently working on optimizing transfection protocols for HEK293 cells using nucleofection. I'm aiming to achieve the highest possible transfection efficiency. Could anyone...
28 May 2024 7,504 3 View
One example article attached
26 May 2024 6,767 2 View
Understanding and Managing Constipation: A Comprehensive Rev... How does the history and physical examination aid in diagnosing constipation, especially in pediatric patients?
25 May 2024 5,806 2 View
I am observing a band at 20kb after ligating a 1.5kb insert with a 4.4kb pCold I plasmid, which should ideally result in a band around 5.9kb (assuming successful ligation). I used BamHI-HF (NEB)...
21 May 2024 9,636 3 View
Hi everyone, I'm currently working on a project where we are using a Jetson Nano in a car, supported by a Waveshare UPS Module B for battery backup. To charge the UPS Module, we need a supply of...
20 May 2024 177 3 View
hi everyone I have synthesized ZnO nanoparticles with different morphologies but I have problem with keeping them dispersed in well diffusion antibacterial method. the results are unreliable...
19 May 2024 9,337 4 View
I am working with primary HUVEC cultures from PromoCell. They proliferate well using ECGM media with 2% FBS from PromoCell (Ref. C-22010), and the cells look healthy and grow successfully. Then,...
15 May 2024 348 1 View
How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...
29 April 2024 1,494 0 View
I'm trying to perform the sulphu-phospho-vanillin assay for total lipid content in microalgae. I'm having trouble with the methodology which I'm not able to understand well when it comes to the...
26 April 2024 6,907 0 View
for example thermal conductivity in deform software is N/sec/C the actual one W/mk heat capacity is N/mm2/C while actual is j/g'C can someone help me through this.
24 April 2024 9,361 1 View
I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...
17 April 2024 2,036 2 View
most of my articles and research are in Arabic. They range from 600 words to 6000 words. I also have books in Arabic and Spanish that could be added. Thank you so much
15 April 2024 2,859 0 View
Developmental stages have a significant impact on anesthesia management in pediatric patients due to physiological, anatomical, and psychological differences that evolve with age.
12 April 2024 2,836 1 View
In the study entitled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan, et,al. (2023), what additional...
12 April 2024 5,941 0 View
É por causa que é pouca à quantidade de sangue amostrada ou alguma enzima digestiva?
12 April 2024 1,093 0 View
In this figure, a pca plot is shown for different protein , protein complex systems depicting the essential subspace for these systems. I want to know how can I interpret these plots ? What...
01 April 2024 9,930 3 View
I am interested in knowing if there is any precedent in the scientific literature on the use of plant growth-promoting bacteria obtained from the rhizosphere that have been inoculated into the...
25 March 2024 6,503 2 View
I am trying to understand the pressure leaders might feel, when a project fails. It is undoubtedly a moment when we should, stop-rethink-process-and move on. But often times since a large amount...
19 March 2024 1,190 0 View
I want to image via 2-photon microscopy immune cells after an Osteotomy. I will adoptively transfer murine monocytes and macrophages. What would be a good number to transfer/cell type? And has...
15 March 2024 8,858 0 View
Currently I am trying abcam protocol for it.i checked the cell lysis by trypan blue stain which is more than 90 %. but I am facing following issues 1. unable to remove beta actin in mitochondrial...
11 March 2024 7,431 1 View