Why didnt methanol boil at 90 degree celcius ?

i extracting the phytochemicals from the grounded root samples using methanol as solvent by soxhlet apparatus. i used oil bath to heat the methanol since my heating mantle wasn't available at the...

03 April 2019 2,451 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

01 February 2019 7,351 6 View

Is it necessary to dissolve the methanolic extract in DMSO before checking the antifungal activty of any plant extract?

so, basically i had to do an anti-fungal assay using a plant extract which was extracted using methanol. then i dissolved the extract in methanol to bring 200mg/ml working concentration. I made...

01 February 2019 5,582 5 View

Can somebody please suggest me with an article to assess the antagonistic activity of a fungi on another fungi ?

i am currently looking for some protocols to be followed to assess the antifungal activity of a new fungus against a pathogenic fungi. i have no idea what fungi it is. so, i dont know what type of...

01 February 2019 3,163 17 View

What does the superscripted alphabet in the data table represent in some research articles ?

recently i encountered some research papers where they have super-scripted some alphabets on top of the values. can somebody please explain me what they are trying to infer. i have attached the...

01 February 2019 2,277 0 View

Is it ok to use McFarland turbidity standards for MIC determination of a plant extract against fungi ?

I have prepared a fungal suspension by scrapping out the fully grown fungal culture from the agar plate and homogenised them. i refered some of the literatures where they have used McFarland...

01 February 2019 3,762 3 View

Can someone help me with GC-MS results analysis?

i have recently done a GCMS analysis for the methanolic extracts of plant samples. i am not sure how to interpret the results. can someone help me with any protocol or procedure on how to begin...

01 February 2019 9,491 8 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

11 December 2018 1,622 6 View

Does nitrocellulose membrane that is used for the southern blotting technique have more binding capacity than the Silica matrix of Dna purification ?

the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does...

07 August 2018 804 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View