Sarath Nair

10 Questions 21 Answers 0 Followers

Questions related from Sarath Nair

Why didnt methanol boil at 90 degree celcius ?

i extracting the phytochemicals from the grounded root samples using methanol as solvent by soxhlet apparatus. i used oil bath to heat the methanol since my heating mantle wasn't available at the...

04 April 2019 2,529 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

02 February 2019 7,433 6 View

What does the superscripted alphabet in the data table represent in some research articles ?

recently i encountered some research papers where they have super-scripted some alphabets on top of the values. can somebody please explain me what they are trying to infer. i have attached the...

02 February 2019 2,357 0 View

Can someone help me with GC-MS results analysis?

i have recently done a GCMS analysis for the methanolic extracts of plant samples. i am not sure how to interpret the results. can someone help me with any protocol or procedure on how to begin...

02 February 2019 9,588 8 View

Is it necessary to dissolve the methanolic extract in DMSO before checking the antifungal activty of any plant extract?

so, basically i had to do an anti-fungal assay using a plant extract which was extracted using methanol. then i dissolved the extract in methanol to bring 200mg/ml working concentration. I made...

02 February 2019 5,663 5 View

Is it ok to use McFarland turbidity standards for MIC determination of a plant extract against fungi ?

I have prepared a fungal suspension by scrapping out the fully grown fungal culture from the agar plate and homogenised them. i refered some of the literatures where they have used McFarland...

02 February 2019 3,847 3 View

Can somebody please suggest me with an article to assess the antagonistic activity of a fungi on another fungi ?

i am currently looking for some protocols to be followed to assess the antifungal activity of a new fungus against a pathogenic fungi. i have no idea what fungi it is. so, i dont know what type of...

02 February 2019 3,251 17 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

12 December 2018 1,717 6 View

How to grow both bacteria and a fungi together in a single media ?

Basically, i am trying to find the antagonistic activity of the bacteria and a pathogenic fungi. what could be the possible way to culture them if the incubation temp is 37 degree celsius for the...

10 October 2018 1,154 2 View

Does nitrocellulose membrane that is used for the southern blotting technique have more binding capacity than the Silica matrix of Dna purification ?

the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does...

08 August 2018 957 4 View