Stefano Falone

10 Questions 31 Answers 0 Followers

Questions related from Stefano Falone

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

25 June 2019 3,279 2 View

Non-specific antibody binding or detection of complexed/immature forms of GSTA2?

Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...

31 October 2017 6,258 6 View

Did anyone find MnSOD (SOD2) in the nucleus?

Hi everyone. I am facing a very intriguing phenomenon. My immunofluorescence and confocal microscopy analyses showed a nucleus that is positive for MnSOD (which should be present only within...

23 December 2016 4,069 12 View

Does anyone know the rate of disappearance of H2O2 in an acqueous solution?

Hi. I know that hydrogen peroxide undergoes spontaneous decomposition when dissolved in an acqueous solution. According to some literature I found, H2O2 would have a half-life of ~5-10 hrs,...

25 July 2016 7,200 6 View

Possible non-parametric alternative to a three-way ANOVA. Any suggestion?

Hi,I have just carried out an experiment in which I measured a dependent variable in cells that were divided in 8 groups. +Magnetic field, +NAC, +H2O2 +Magnetic field, -NAC, +H2O2 +Magnetic...

15 April 2016 3,869 18 View

Does anyone know a specific membrane-permeable MnSOD inhibitor?

Hi everyone,I am looking for a specific chemical inhibitor of MnSOD (SOD2). It should be able to pass the plasma membrane freely. Available literature is not helping. Thank You very much in...

30 March 2016 3,082 4 View

Weird phenomenon: spontaneous Western blot revelation and brown band appearance. How could that be possible?

Hi everyone, a very weird phenomeon is occurring in my lab. In order to detect some proteins that are expressed at low level I am using an ECL kit (for high level-expressed proteins I normally use...

16 March 2016 2,443 12 View

How can I proceed for extensive protein hydrolysis and MG-H1 determination?

Hi,I am trying to measure the content of methylglyoxal-dependent damage on arginine residues in my protein samples. I know an extensive protein hydrolysis must be carried out in order to...

18 March 2015 8,818 2 View

Can anyone help me with a problem I encounter on some of my cDNAs samples (from mouse oocytes), when I perform SYBRgreen-based real time PCRs?

I get no amplification but I see a very high fluorescence (normal ROX profile) already before the first PCR cycle (raw data: 1x10^6 fluo units). Such a high fluorescence declines...

12 September 2014 1,829 4 View

Sirtuinome Rewiring to Hijack Cancer Cell Behavior and Hamper Resistance to Anticancer Intervention

In collaboration with Frontiers in Oncology (latest Clarivate Analytics' impact factor: 4.416), "Cancer Metabolism" section, and together with Professors Athanassios Vassilopoulos and Lucia...

01 January 1970 9,428 4 View