18 Questions 61 Answers 0 Followers
Questions related from Stefano Falone
Hi everyone. I am interested in removing efficiently from media (DMEM high glucose w/o phenol red+glutamine+P/S) conditioned by commercially available primary human adipose tissue mesenchymal stem...
24 May 2025 2,131 2 View
Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...
25 June 2019 3,388 2 View
Hi everyone. I needed a mitochondrial marker for immunofluorescence co-localizazion analyses. I decided to use the Thermo Fisher Scientific MitoTracker (cat. no. M7510). I stained HUVECs with 100...
23 May 2018 6,099 35 View
Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...
31 October 2017 6,311 6 View
Hi everyone. I am facing a very intriguing phenomenon. My immunofluorescence and confocal microscopy analyses showed a nucleus that is positive for MnSOD (which should be present only within...
23 December 2016 4,119 12 View
Hi. I know that hydrogen peroxide undergoes spontaneous decomposition when dissolved in an acqueous solution. According to some literature I found, H2O2 would have a half-life of ~5-10 hrs,...
25 July 2016 7,250 6 View
Hi,I have just carried out an experiment in which I measured a dependent variable in cells that were divided in 8 groups. +Magnetic field, +NAC, +H2O2 +Magnetic field, -NAC, +H2O2 +Magnetic...
15 April 2016 3,921 18 View
Hi,my experiments are trying to show that the elevation of mitochondrion-generated H2O2 acts as a trigger for the initiation of a certain phenomenon, hence I would like to trap specifically...
06 April 2016 2,231 11 View
Hi everyone,I am looking for a specific chemical inhibitor of MnSOD (SOD2). It should be able to pass the plasma membrane freely. Available literature is not helping. Thank You very much in...
30 March 2016 3,137 4 View
Hi,I have to find a specific chemical activator of glyoxalase 1 (GLO1). It should have the capacity to pass the cell membrane roughly freely. Thank You very much in advance. Regards Stefano...
30 March 2016 6,141 2 View
Hi everyone, a very weird phenomeon is occurring in my lab. In order to detect some proteins that are expressed at low level I am using an ECL kit (for high level-expressed proteins I normally use...
16 March 2016 2,515 12 View
Hi,I know that total protein amount may be a good marker of in vitro biomass (see references below), however I am looking for papers that demonstrate a good correlation between cell number in...
10 February 2016 3,041 2 View
I need to verify whether my treatment may alter the invasive properties of a human neuroblastoma cell line. I have a PerkinElmer Victor 3 microplate reader. Does anyone know a reliable and...
04 June 2015 8,370 3 View
Hi,I am trying to measure the content of methylglyoxal-dependent damage on arginine residues in my protein samples. I know an extensive protein hydrolysis must be carried out in order to...
18 March 2015 8,885 2 View
I get no amplification but I see a very high fluorescence (normal ROX profile) already before the first PCR cycle (raw data: 1x10^6 fluo units). Such a high fluorescence declines...
12 September 2014 1,887 4 View
In collaboration with Frontiers in Oncology (latest Clarivate Analytics' impact factor: 4.416), "Cancer Metabolism" section, and together with Professors Athanassios Vassilopoulos and Lucia...
01 January 1970 9,477 4 View
Open Special Issue of Hindawi's Oxidative Medicine and Cellular Longevity titled "OxidativeStress and Reprogramming of Mitochondrial Function and Dynamics as Targets to Modulate Cancer Cell...
01 January 1970 4,879 0 View
Hi everyone. My cultivated tumor granulosa cells are undergoing a senescence-inducing treatment. At the end of it, I am counting SA-beta-gal+ cells, so I want to counterstain nuclei with...
01 January 1970 3,209 0 View
