How to exclude direct effect of drugs to neurons in mouse parkinson's model?

26 October 2020 1,127 2 View

LC3I band detection?

Hi, I have some questions about the electrophoresis and transfer condition. In our lab, we used 15% gel to SDS-page and semi-dry transfer In electrophoresis, i migrated the stacking gel in 80 V...

14 October 2020 4,046 2 View

How do I detect sample cross-contamination in a batch of SNP array data?

I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...

06 March 2020 7,896 3 View

How can i solve this problem, OPV active film getting hazy?

Hi, guys.. These days, I have some problem in OPV processing. To be more specific, the active film is OK right after spin casting. But while drying it, the film is getting hazy..... it is changed...

07 January 2020 7,673 4 View

How to make SnO2 thin film by SnCl2-2H2O?

26 November 2019 5,407 3 View

What size of gold nanoparticles should I synthesize?

13 July 2019 5,878 2 View

how high temperature pepper fruits can resist?

I hope to monitor how fungal conidia (colletotrichum acutatum) infect on dead pepper fruits. so, I dipped sliced fragments of a pepper fruit in boiling water for 30 seconds and inoculated fungal...

11 July 2019 6,618 3 View

Is it appropriate to use DMSO:Corn oil (50:50) in mouse as IP?

Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...

03 December 2018 4,092 1 View

Where is the proper cooling rate measurement position in the transverse-varestraint test(GTWA)?

Maximum crack length and cooling rate measurements are required to calculate the BTR from the transverse-varestraint test. In previous studies, it is stated that the weld pool should be measured...

13 November 2018 9,486 1 View

Do you know reason for wrong output on HOMO and LUMO energies in Gamess?

In the output result, I found that HOMO and LUMO energies were too high. I wonder what is the problem in here. Please check attached files. In first file, NPREO was used to print homo-1, homo,...

23 August 2018 7,738 7 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Do the freezing reduces the efficacy of the cells for the electroporation ? should i decrease the voltage for the next time?

I'm making reprogramming of MEFs experiment and I used Piggy bac, Sleeping beauty and episomal transposition systems. After electroporation i froze the cells, then i thawed the cells but there...

06 January 2021 5,467 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

What is the best dye to use to visualise electroporation of neurons with DNA for later expression of a genetically-encoded fluorescent reporter?

We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...

17 November 2020 2,794 1 View

How can I simulate electroporation for a bilayer?

hi I am trying to simulate electroporation for a bilayer containing 10% cholesterol on GROMACS. I have read lots of articles but the only parameter that they have talked about was the electric...

15 November 2020 7,602 2 View

Can I do RNA electroporation without cleanup after in vitro transcription?

I am working on an EMCV like virus with about 8kbp genome. The RNA is usually prepared from digested plasmids by in-vitro transcription, followed by DNase I digestion, followed by P/C and...

02 November 2020 442 3 View

Hi all, I need to transform a reporter plasmid into a mutant of Mycobacterium avium hominissuis which has the same antibiotic marker as the plasmid?

I tried to electroporate and select the colonies by plating onto a higher concentration of antibiotic. But it did not work!

20 September 2020 6,458 3 View