How to exclude direct effect of drugs to neurons in mouse parkinson's model?

26 October 2020 1,127 2 View

LC3I band detection?

Hi, I have some questions about the electrophoresis and transfer condition. In our lab, we used 15% gel to SDS-page and semi-dry transfer In electrophoresis, i migrated the stacking gel in 80 V...

14 October 2020 4,046 2 View

How can i solve this problem, OPV active film getting hazy?

Hi, guys.. These days, I have some problem in OPV processing. To be more specific, the active film is OK right after spin casting. But while drying it, the film is getting hazy..... it is changed...

07 January 2020 7,673 4 View

How to make SnO2 thin film by SnCl2-2H2O?

26 November 2019 5,407 3 View

What size of gold nanoparticles should I synthesize?

13 July 2019 5,878 2 View

how high temperature pepper fruits can resist?

I hope to monitor how fungal conidia (colletotrichum acutatum) infect on dead pepper fruits. so, I dipped sliced fragments of a pepper fruit in boiling water for 30 seconds and inoculated fungal...

11 July 2019 6,618 3 View

Is it appropriate to use DMSO:Corn oil (50:50) in mouse as IP?

Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...

03 December 2018 4,092 1 View

Do you know reason for wrong output on HOMO and LUMO energies in Gamess?

In the output result, I found that HOMO and LUMO energies were too high. I wonder what is the problem in here. Please check attached files. In first file, NPREO was used to print homo-1, homo,...

23 August 2018 7,738 7 View

Has anybody tried the cellulose-based DNA clean-up method?

Hello colleagues, Nucleic acid purification from plants, animals and microbes ... I wonder if this method works in other people's hands, particularly for adherent mammalian cells. I also wonder...

04 May 2018 3,729 0 View

The usage of clock circuit in micro controller chip?

Why do we use clock circuit when we are designing certain micro controller onto PCB? What it is usage? What other circuits do we need in order to proceed? Would you be able to give some advice...

03 January 2018 8,975 12 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View