7 Questions 7 Answers 0 Followers
Questions related from Daiha Shin
Is it okay to use liquid argon as an inert gas in a glove box? I'm currently using argon gas, and it needs frequent replacements, which is inconvenient. I'm wondering if using liquid argon might...
13 December 2023 9,103 2 View
Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...
04 December 2018 4,223 1 View
We use cells as doing subculture (ex: cancer cells). However I don't know of the timing changing the cells. When is the timing changing the cell ?
27 October 2017 4,626 6 View
What is standard of resistant cell lines by specific drug ? I wonder if IC50 of resistant cell line is more 4 times than of original cell line, can the former be resistant cell line? (resistant...
26 May 2017 8,733 2 View
If drug inducing ROS or lipid ROS is treated dose-dependently in specific cells, level of ROS measurement by FACS increased dose-dependently?
22 May 2017 7,040 4 View
What are different positive controls in experiment measuring lipid ROS using BODIPY(581/591)?
17 May 2017 866 2 View
30 ug protein Loading -> Transfer 300mA/1h PVDF 1. 5% BSA blocking 1hr 2. 1st antibody (pBeclin-1) 0.5ug/ml dilution in 2.5% BSA, O/N 3. TBS-T wash 5/5/5/10 min 4. 2nd antibody (rabbit) 0.2...
17 October 2016 232 3 View