How can i express my data as percent of control in western blot?

i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance

10 November 2018 9,245 0 View

I want to prepare sample for western blot technique and determine phosphorylated protein .can somone give me a good protocol for prepation of sample ?

can somone give me a good protocol for prepation of sample from rat brain tissue?

05 June 2018 4,682 0 View

What are the factor should i considered when choose age and weight of rat in my experiment?

i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...

31 December 2017 5,698 5 View

Is the dose in mice equal the dose in rat except body weight or there is equation to convert dose from mice to rat?

i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?

31 December 2017 9,588 10 View

Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?

Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?

31 December 2017 10,018 3 View

What is the differnt between Milli-Q water and water for injection?

i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...

31 December 2017 841 7 View

Can i use 20000 rpm in separating mitochondria of rat brain instead of 21000 rpm ?

in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...

31 December 2017 1,310 2 View

Can any one simplify method of TTC staining of the brain to determine infarction volume ?

can any one simplify method of TTC staining of the brain to determine infarction volume and area?

31 December 2017 6,715 3 View

If the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

31 December 2017 2,299 6 View

I want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

31 December 2017 381 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View