can somone give me a good protocol for prepation of sample from rat brain tissue?
i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance
10 November 2018 9,245 0 View
some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...
01 February 2018 9,994 0 View
i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...
31 December 2017 5,698 5 View
i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?
31 December 2017 9,588 10 View
Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?
31 December 2017 10,018 3 View
i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...
31 December 2017 841 7 View
in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...
31 December 2017 1,310 2 View
can any one simplify method of TTC staining of the brain to determine infarction volume and area?
31 December 2017 6,715 3 View
if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?
31 December 2017 2,299 6 View
i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?
31 December 2017 381 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View