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Questions related to pH
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
13 August 2024 842 0 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
06 August 2024 8,926 3 View
I'm working on carboplatin which i bought from local pharmacy. It is a Carboplatin injection IP 150mg/15ml which is sterile aqueous solution for intravenous use. Unfortunately, I cannot able to...
05 August 2024 432 3 View
I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.
04 August 2024 3,144 1 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
03 August 2024 9,483 2 View
This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...
03 August 2024 4,728 3 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
02 August 2024 9,197 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
01 August 2024 200 2 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
01 August 2024 8,632 3 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
01 August 2024 2,130 0 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
31 July 2024 2,458 5 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
30 July 2024 5,593 3 View
There are three operating parameters - the laser power, the diameter of the focus spot and the scanning speed. It is necessary to obtain a seam of a given depth without pores. What parameter...
28 July 2024 7,145 2 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
27 July 2024 1,446 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
26 July 2024 7,472 2 View
What role do microorganisms play in the cycling of matter and role of microbes in the biogeochemical cycle?
26 July 2024 3,067 2 View
I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?
24 July 2024 5,736 2 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
24 July 2024 3,868 0 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
24 July 2024 9,399 3 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
24 July 2024 9,892 1 View
How can we improve resilience to climate change and role of climate-smart agriculture in enhancing farmers livelihood and sustainable forest management?
23 July 2024 3,321 5 View
What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
23 July 2024 5,305 6 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
23 July 2024 8,741 1 View
How does rainwater harvesting increase the groundwater levels and recharging of groundwater by making the rainwater to populate into the soil?
22 July 2024 4,000 2 View