How can i express my data as percent of control in western blot?

i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance

10 November 2018 9,245 0 View

I want to prepare sample for western blot technique and determine phosphorylated protein .can somone give me a good protocol for prepation of sample ?

can somone give me a good protocol for prepation of sample from rat brain tissue?

05 June 2018 4,682 0 View

What is the most suitable solution should be perfused to the rat heart before decapitation in order to get clear immunohistochemistry pictures ?

some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...

01 February 2018 9,994 0 View

What are the factor should i considered when choose age and weight of rat in my experiment?

i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...

31 December 2017 5,698 5 View

Is the dose in mice equal the dose in rat except body weight or there is equation to convert dose from mice to rat?

i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?

31 December 2017 9,588 10 View

What is the differnt between Milli-Q water and water for injection?

i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...

31 December 2017 841 7 View

Can i use 20000 rpm in separating mitochondria of rat brain instead of 21000 rpm ?

in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...

31 December 2017 1,310 2 View

Can any one simplify method of TTC staining of the brain to determine infarction volume ?

can any one simplify method of TTC staining of the brain to determine infarction volume and area?

31 December 2017 6,715 3 View

If the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

31 December 2017 2,299 6 View

I want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

31 December 2017 381 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View