i done western blot for phosphorylated protein and total protein
how can i make graph represent result of western blot as percent of control?
thanks in advance
can somone give me a good protocol for prepation of sample from rat brain tissue?
05 June 2018 4,682 0 View
some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...
01 February 2018 9,994 0 View
i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...
31 December 2017 5,698 5 View
i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?
31 December 2017 9,588 10 View
Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?
31 December 2017 10,018 3 View
i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...
31 December 2017 841 7 View
in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...
31 December 2017 1,310 2 View
can any one simplify method of TTC staining of the brain to determine infarction volume and area?
31 December 2017 6,715 3 View
if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?
31 December 2017 2,299 6 View
i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?
31 December 2017 381 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...
12 February 2021 5,824 3 View