i done western blot for phosphorylated protein and total protein
how can i make graph represent result of western blot as percent of control?
thanks in advance
can somone give me a good protocol for prepation of sample from rat brain tissue?
05 June 2018 4,781 0 View
some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...
01 February 2018 10,078 0 View
i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?
31 December 2017 9,737 10 View
i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...
31 December 2017 5,800 5 View
Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?
31 December 2017 10,122 3 View
i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...
31 December 2017 1,129 7 View
can any one simplify method of TTC staining of the brain to determine infarction volume and area?
31 December 2017 6,812 3 View
in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...
31 December 2017 1,411 2 View
if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?
31 December 2017 2,398 6 View
i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?
31 December 2017 454 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...
15 July 2024 1,940 3 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View