Can any one simplify method of TTC staining of the brain to determine infarction volume ?

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How can i express my data as percent of control in western blot?

i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance

10 November 2018 9,362 0 View

I want to prepare sample for western blot technique and determine phosphorylated protein .can somone give me a good protocol for prepation of sample ?

can somone give me a good protocol for prepation of sample from rat brain tissue?

05 June 2018 4,781 0 View

What is the most suitable solution should be perfused to the rat heart before decapitation in order to get clear immunohistochemistry pictures ?

some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...

01 February 2018 10,078 0 View

Is the dose in mice equal the dose in rat except body weight or there is equation to convert dose from mice to rat?

i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?

31 December 2017 9,737 10 View

What are the factor should i considered when choose age and weight of rat in my experiment?

i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...

31 December 2017 5,800 5 View

Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?

31 December 2017 10,122 3 View

What is the differnt between Milli-Q water and water for injection?

i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...

31 December 2017 1,129 7 View

Can i use 20000 rpm in separating mitochondria of rat brain instead of 21000 rpm ?

in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...

31 December 2017 1,411 2 View

If the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

31 December 2017 2,398 6 View

I want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

31 December 2017 454 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View