Can any one simplify method of TTC staining of the brain to determine infarction volume ?

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How can i express my data as percent of control in western blot?

i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance

10 November 2018 9,245 0 View

I want to prepare sample for western blot technique and determine phosphorylated protein .can somone give me a good protocol for prepation of sample ?

can somone give me a good protocol for prepation of sample from rat brain tissue?

05 June 2018 4,682 0 View

What is the most suitable solution should be perfused to the rat heart before decapitation in order to get clear immunohistochemistry pictures ?

some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...

01 February 2018 9,994 0 View

What are the factor should i considered when choose age and weight of rat in my experiment?

i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...

31 December 2017 5,698 5 View

Is the dose in mice equal the dose in rat except body weight or there is equation to convert dose from mice to rat?

i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?

31 December 2017 9,588 10 View

Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?

31 December 2017 10,018 3 View

What is the differnt between Milli-Q water and water for injection?

i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...

31 December 2017 841 7 View

Can i use 20000 rpm in separating mitochondria of rat brain instead of 21000 rpm ?

in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...

31 December 2017 1,310 2 View

If the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

31 December 2017 2,299 6 View

I want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

31 December 2017 381 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View