Salma Nabil

15 Questions 18 Answers 0 Followers

Questions related from Salma Nabil

How can i express my data as percent of control in western blot?

i done western blot for phosphorylated protein and total protein how can i make graph represent result of western blot as percent of control? thanks in advance

11 November 2018 9,323 0 View

I want to prepare sample for western blot technique and determine phosphorylated protein .can somone give me a good protocol for prepation of sample ?

can somone give me a good protocol for prepation of sample from rat brain tissue?

06 June 2018 4,746 0 View

What is the most suitable solution should be perfused to the rat heart before decapitation in order to get clear immunohistochemistry pictures ?

some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M...

02 February 2018 10,052 0 View

I want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

i want to determine infarct volume and area of the brain using crystal violet staining,can any one give me link for download image j software?

01 January 2018 437 2 View

Is the dose in mice equal the dose in rat except body weight or there is equation to convert dose from mice to rat?

i want to test neuroprotection of drug in rat but the dose present of this drug in mice only so what should i do?

01 January 2018 9,703 10 View

If the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

if the drug pass blood brain barrier in mouse,can i consider that it will pass blood brain barrier in rat ?

01 January 2018 2,366 6 View

Is there a difference in how drugs cross the BBB with ip versus subcutenous injections?

01 January 2018 10,091 3 View

What are the factor should i considered when choose age and weight of rat in my experiment?

i will do my research which is testing neuroprotection of drug onglobal cereberal ischemia reperfusion model in wistar rat but i want to know the factor should i considered when i choose age and...

01 January 2018 5,775 5 View

Can any one simplify method of TTC staining of the brain to determine infarction volume ?

can any one simplify method of TTC staining of the brain to determine infarction volume and area?

01 January 2018 6,784 3 View

What is the differnt between Milli-Q water and water for injection?

i will use this water to prepare stock solution of my pepptide and when i red papers i found that they dissolve this peptide in milli-q water so i asking if i can use water for injection instead...

01 January 2018 1,088 7 View

What is the difference between pharmaceutical grade and analytical grade chemical?

what is the difference between pharmaceutical grade and analytical grade chemical?

01 January 2018 4,469 0 View

Can i use 20000 rpm in separating mitochondria of rat brain instead of 21000 rpm ?

in my lab the highest speed of centrifuge is 20000 rpm but the method of separation mitochondria of rat brain contain centrifuge has 21000 rpm ,so can i use 20000 rpm instead of 21000 rpm in...

01 January 2018 1,384 2 View

How many days should i give the drug to test its ability for neuroprotection in diabetic rat and have global cereberal ischemia reperfusion injury?

i want to know if i want to test drug for its neuroprotection effect how many days should i give the drug for rat to test its ability for neuroprotection?

12 December 2017 5,749 2 View

From where i can buy storoscopic illumination gives 300 flash per minute as type of stress in chronic mild stress model in rat ?

I am from egypt and i want to know feom where i can buy storoscopic illumination gives 300 flash per minute

11 November 2017 3,876 2 View

Which company is more better to purchase specific drug from it Medkoo Bioscience or Medchem express or Adooq or APExBIO ?

which company is more better

11 November 2017 4,899 1 View