Can anyone recomend a "surplus" lab equipment sales company for our thin-film PVD system?

We are scaling down our thin film R&D projects, and are looking to sell off / find a good home for our Thin-film & Nanoparticle Physical Vapour Deposition system. I was wondering if...

16 September 2020 6,550 3 View

Where to find OnLine Volunteer jobs, solving Algebraic thru Ordinary Differential Equations, in the Math Modeling, Simulation, & Optimization arenas?

I'm a retired applied mathematician seeking a volunteer job using my (math/EE) education and experience in (disc drive/airplane/space) industries. A calculus-level compiler provides a quick way...

16 February 2020 2,394 3 View

How to remove carry-over in LC-MS/MS gradient method when isocratic method shows no sign of carry-over

I have a new molecule for which I have initially developed an isocratic LC-MS/MS method (Synergi Fusion column, H2O/ACN 20/80 (+0.1% HCOOH)). Analysis of this method gives limited sensitivity...

25 June 2017 1,721 11 View

When using the 'Concrete damaged plasticity' model in ABAQUS, can you view the plastic crack widths that the program is calculating?

Refering to section '18.5.3 Concrete damaged plasticity' in the ABAQUS manual, there is a parameter L0 for cracked damage. Can you output this value? I am getting warnings about negative increases...

10 June 2015 1,092 2 View

Technology to probe physics of polymer brushes?

Do you think the technology described in this article could be used to look at the conformation / physics of tethered polymers (both neutral and polyeletrolytes) under static and shear flow...

02 December 2014 5,813 5 View

Can anyone help with correlation of qPCR and western blot reults?

I knocked down a gene which has been shown to promote cell proliferation. qPCR results confirmed that the gene was indeed knocked down. There was increased expression of p53 as a result, which...

24 September 2014 9,241 5 View

Why are my no template controls for qPCR yielding products?

My "no template" controls are showing products that are identical to their genes. I did bax and p53 qPCR and noticed that bax amplification was almost identical to bax no template control; p53,...

26 May 2014 6,402 7 View

Would any MDs/PhDs be willing to be interviewed as part of a neuroethnographic research project? I want to hear your thoughts on TBI/PTSD treatments.

First and foremost - I cannot actually begin to formally collect data until June of 2014, as I will have to meet with an IRB to both receive approval and to establish how to best ensure research...

14 January 2014 8,525 5 View

Hi, I keep getting very low yields of RNA from my RNA extractions

I am using duplicate wells per sample in a 24 well plate. I had no problem when I extracted total RNA from a culture flask. I am using a Nucleospin extraction kit from Promega. I get low yields...

03 September 2013 2,192 22 View

Can I use RNAse-free water in the place of 'nuclease-free' water?

Can I use RNAse-free water in the place of 'nuclease-free' water? Is RNase-free water always DNase-free OR is DNase -free water always RNase free?? The term nuclease-free water seems all...

30 July 2013 790 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

How to make a site-Selective Electroless Nickel Plating?

I would like to plating Ni/Au on the selected area. Can anyone give me a suggestion? It is a bit like to make golden finger on PCB board. Instead with the PCB board, I use polyimide as my...

28 January 2021 8,330 1 View

How do I dissolve cannabidiol (CBD) and crude hemp extracts in 1:1:18 ethanol:tween80:0.9% saline for IP injection in mice?

I have tried dissolving CBD and crude hemp extracts in 1:1:18 ethanol: tween80: 0.9% saline. I dissolved the drugs in ethanol first then tween, after 10 mins sonication the solutions looks ok but...

27 January 2021 6,259 3 View