Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

No title

28 February 2020 1,633 2 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

On a EVO LS10 SEM, I can see images on spot 0 of the aperture, however on spot 1 and 2, I cant see any images. what could be responsible for this?

I an using the EVO LS10 SEM from Zeiss, I can see images on spot 0 of the aperture, however when the aperture is switched to spot 1 or 2, I am unable to see any image. Please what could be...

05 February 2019 3,902 1 View

Where to get yeast W303-K6001 strain?

15 September 2018 3,735 2 View

My solvent-casted PVDF film was opaque and somehow shrunk during vacuum drying. What should I do?

I'm trying to make smooth PVDF film using solvent casting dried under vacuum from 20 mL of 15% PVDF in DMF solution. The vacuum dryer temperature was at 55 deg C. After some time, this is what I...

29 January 2018 803 4 View

How can I get rid of the polysaccharides on orchids tubers to isolate DNA?

I try to isolate DNA from orchids tubers, the problem is they become kind of gelatinous because the polysaccharides.... so I want to get rid of them. Thank you!

18 September 2017 9,815 3 View

Shuold the "Reads" be included or taken into consideration for calculating the researcher's score in RG?

Any RG's member

02 March 2017 6,338 8 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View

What is the main difference between "CD105+ Endothelial Cells" and mesenchymal stem cells?

since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot

03 February 2021 5,362 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View