8 Questions 3 Answers 0 Followers
Questions related from Givon Sanati
I am trying to preserve a population of glioblastoma cells that I believe to be cancer stem cell. I wish to passage the cells and preserve their "stemness" during each passage. I need the right...
05 May 2016 1,697 2 View
I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.
01 January 2016 7,029 3 View
I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...
01 January 2016 9,487 2 View
I am trying to separated stem cells from old differentiated senescent to enrich a population of my culture. Stem cells are usually in a quiescent state, so to separate would be ideal for me.
12 December 2015 7,481 5 View
I am culturing this U87 cell line and I was wondering what is the recommended media. I have read the literature, but they have been giving conflicting answers. Also, if there is any extra advice...
10 October 2015 9,192 3 View
I am try to find a way to differentiated the cancer cells to a point where they are terminally differentiated. They are similar to Neural stem cells which is why I removed all of the growth...
10 October 2015 8,441 2 View
I am trying to understand whether cancer stem cells are the metastatic cells in cancer progression because of their ability to self-renew and proliferate on a single cell level.
10 October 2015 4,394 0 View
In the NSC media I use neurobasal medium (life technology) b27 supplement, 20 ng/mL human FGF-2 (Peprotech), 20 ng/mL human EGF (Promega), and 40 μg/mL heparin (Sigma). Glioblastoma Medium is N2...
08 August 2015 9,353 1 View
