In the NSC media I use neurobasal medium (life technology) b27 supplement, 20 ng/mL human FGF-2 (Peprotech), 20 ng/mL human EGF (Promega), and 40 μg/mL heparin (Sigma).
Glioblastoma Medium is N2 medium, which contains DMEM/F-12 (Omega Scientific), 1% N2 supplement (Invitrogen), 20 ng/mL human FGF-2 (Peprotech), 20 ng/mL human EGF (Promega), and 40 μg/mL heparin (Sigma)
Noureldien Darwish
depend on the type of glioblastoma cell line, for example (u-87 luc) is a very aggressive cell line that can grow with any media in short time
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