What is the best value luminescence/fluorescence microplate reader on the market?

07 August 2019 1,289 3 View

How is detection of E-cadherin in osteosarcoma cell lines U20S or Saos2?

We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...

03 April 2018 9,717 1 View

Human cell line overexpressing DNMT1

04 May 2017 4,818 0 View

MiRNA expression and knock-down in nude mice

04 May 2017 1,084 4 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

01 February 2017 7,296 1 View

MIgration assay with U2OS, are there any tricks?

Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...

08 September 2016 5,014 4 View

Does anyone know of a SP1 null cell line?

11 December 2014 3,171 0 View

Does anyone know if there is company which sells antibodies against Ehrlichia species?

I need antibodies for FACS experiments using ehrlichia infected cell culture cells.

02 March 2013 9,819 2 View

I am about to start a new project with canine cells. In my lab we have DH82, D17 and MDCK. Can anyone provide more lines?

Canine cells for a research projetct

31 December 2012 6,806 0 View

Which science project manager software is the best in your opinion, please?

Looking for a project manager software for jy lab

11 December 2012 7,245 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View