I am looking for a pair of cell lines SP1+/+ and SP1-/-
I am out in the market for a luminescence/fluorescence microplate reader. I have quotes for the Promega GloMax® Explorer and the Synergy Lx, Multi-mode Reader. Please, can anyone comment on these...
07 August 2019 1,389 3 View
We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...
03 April 2018 9,791 1 View
Please, does anyone know of a human cell line with overexpression of DNMT1? Or even better, a cell line with tet on system for overexpression of DNMT1? We are also looking for a tet on p53 mutant...
04 May 2017 4,890 0 View
Pease, we are new to the field, and text in manuscripts are sometimes confusing. I want to study the effect of overexpression and down expression of a given miRNA in tumor development. Is it...
04 May 2017 1,164 4 View
When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...
01 February 2017 7,384 1 View
Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...
08 September 2016 5,090 4 View
What are the cases of success versus non success
06 July 2013 6,420 1 View
I need antibodies for FACS experiments using ehrlichia infected cell culture cells.
02 March 2013 9,885 2 View
Canine cells for a research projetct
31 December 2012 6,880 0 View
Looking for a method to study dnmts interaction with microRNAs under different experimental conditions.
11 December 2012 7,660 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
