What is the best value luminescence/fluorescence microplate reader on the market?

07 August 2019 1,289 3 View

How is detection of E-cadherin in osteosarcoma cell lines U20S or Saos2?

We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...

03 April 2018 9,717 1 View

MiRNA expression and knock-down in nude mice

04 May 2017 1,084 4 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

01 February 2017 7,296 1 View

MIgration assay with U2OS, are there any tricks?

Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...

08 September 2016 5,014 4 View

Does anyone know of a SP1 null cell line?

11 December 2014 3,171 0 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

06 July 2013 6,358 1 View

Does anyone know if there is company which sells antibodies against Ehrlichia species?

I need antibodies for FACS experiments using ehrlichia infected cell culture cells.

02 March 2013 9,819 2 View

I am about to start a new project with canine cells. In my lab we have DH82, D17 and MDCK. Can anyone provide more lines?

Canine cells for a research projetct

31 December 2012 6,806 0 View

Which science project manager software is the best in your opinion, please?

Looking for a project manager software for jy lab

11 December 2012 7,245 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View