How is detection of E-cadherin in osteosarcoma cell lines U20S or Saos2?

We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...

03 April 2018 9,717 1 View

Human cell line overexpressing DNMT1

04 May 2017 4,818 0 View

MiRNA expression and knock-down in nude mice

04 May 2017 1,084 4 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

01 February 2017 7,296 1 View

MIgration assay with U2OS, are there any tricks?

Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...

08 September 2016 5,014 4 View

Does anyone know of a SP1 null cell line?

11 December 2014 3,171 0 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

06 July 2013 6,358 1 View

Does anyone know if there is company which sells antibodies against Ehrlichia species?

I need antibodies for FACS experiments using ehrlichia infected cell culture cells.

02 March 2013 9,819 2 View

I am about to start a new project with canine cells. In my lab we have DH82, D17 and MDCK. Can anyone provide more lines?

Canine cells for a research projetct

31 December 2012 6,806 0 View

Which science project manager software is the best in your opinion, please?

Looking for a project manager software for jy lab

11 December 2012 7,245 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View