Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

02 February 2017 1 7K Report

When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a left over of Tp53 sequence?

Mahdi Saber Al-Deresawi

yes you are wright , the real-time with taqman is very specific than the conventional RT-PCR because the detection by conventional RT-PCR depend on the double strand .

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