Mozart Marins

15 Questions 10 Answers 0 Followers

Questions related from Mozart Marins

What is the best value luminescence/fluorescence microplate reader on the market?

I am out in the market for a luminescence/fluorescence microplate reader. I have quotes for the Promega GloMax® Explorer and the Synergy Lx, Multi-mode Reader. Please, can anyone comment on these...

08 August 2019 1,360 3 View

How is detection of E-cadherin in osteosarcoma cell lines U20S or Saos2?

We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...

04 April 2018 9,766 1 View

Human cell line overexpressing DNMT1

Please, does anyone know of a human cell line with overexpression of DNMT1? Or even better, a cell line with tet on system for overexpression of DNMT1? We are also looking for a tet on p53 mutant...

05 May 2017 4,876 0 View

MiRNA expression and knock-down in nude mice

Pease, we are new to the field, and text in manuscripts are sometimes confusing. I want to study the effect of overexpression and down expression of a given miRNA in tumor development. Is it...

05 May 2017 1,134 4 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...

02 February 2017 7,360 1 View

MIgration assay with U2OS, are there any tricks?

Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...

09 September 2016 5,063 4 View

Does anyone know of a SP1 null cell line?

I am looking for a pair of cell lines SP1+/+ and SP1-/-

12 December 2014 3,215 0 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

07 July 2013 6,402 1 View

Does anyone know if there is company which sells antibodies against Ehrlichia species?

I need antibodies for FACS experiments using ehrlichia infected cell culture cells.

03 March 2013 9,869 2 View

I am about to start a new project with canine cells. In my lab we have DH82, D17 and MDCK. Can anyone provide more lines?

Canine cells for a research projetct

01 January 2013 6,852 0 View

Which science project manager software is the best in your opinion, please?

Looking for a project manager software for jy lab

12 December 2012 7,296 2 View

Is FPLC the best way or at least a good and feasible way to purify dnmt1 complexes with other proteins and also nucleic acids, possibly microRNAs?

Looking for a method to study dnmts interaction with microRNAs under different experimental conditions.

12 December 2012 7,649 1 View

Does anyone know if there are methods for quantification of DNA methylation by flow cytometry?

I am writing a project to study DNA methylation during infection and I want to measure global methylation taking advantage of a cell cytometer equipment.

11 November 2012 9,310 6 View

Does anyone have comments on the Cytell Flow Cytometer from GE?

I am doing a project and considering asking for funding to buy one.

11 November 2012 1,674 5 View

Please, does anyone in Brazil cultivate a non cancerous bone cell line such as hFOB 1.19?

I would like to have as a control for my experiments, a non cancerous cell line of bone origin. Hfob is a an alternative but didn´t find someone who cultivates this cell line in Brasil. Thanks for...

01 January 1970 9,565 4 View