Hello everyone,

I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase 3 and 9(active form) . Any recommendations? 

I transfer my proteins to a PVDF membrane for 30 min at 100V. 

Loaded protein amount: 40 microgram. 

THANK YOU. 

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