PD-L1 detection after SDS-PAGE?

Hello, Does anyone have experience with PD-L1 detection by SDS-PAGE? I am using 2 antibodies from Cell signaling Technology and E-bioscience with apposite outcomes. Furthermore, when reprobing...

01 February 2020 8,658 3 View

How to remove EGF from cell culture medium?

Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...

11 December 2018 6,489 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

03 April 2016 4,209 4 View

Rhodamine 123 staining protocol for Flow cytometry?

03 April 2016 6,364 4 View

Detecting active form of caspase 3 and 9 with western blotting?

Hello everyone, I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase...

01 February 2016 8,428 1 View

Treating cancer stem cells with drugs?

31 December 2015 9,346 1 View

Any advice on PI staining and FACS analysis on cancer stem cells?

dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...

11 December 2015 5,270 4 View

Migrating metatstatic cancer clusters?

Hello everyone, I would like to know the composition of migrating metastatic clusters generated by EMT. Any idea, please? Thanks in advance.

10 November 2015 1,670 2 View

How can I stain adherent cells with Annexin V ?

06 July 2015 2,564 2 View

Why am I experiencing high cytotoxicity when transfecting the T24 cell line?

06 July 2015 8,798 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View