PD-L1 detection after SDS-PAGE?

Hello, Does anyone have experience with PD-L1 detection by SDS-PAGE? I am using 2 antibodies from Cell signaling Technology and E-bioscience with apposite outcomes. Furthermore, when reprobing...

01 February 2020 8,658 3 View

How to remove EGF from cell culture medium?

Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...

11 December 2018 6,489 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

03 April 2016 4,209 4 View

Rhodamine 123 staining?

Hello, I am planning to carry on a rhodamine 123 staining to measure the activity of multi drug resistant protein on cells after treatment with a drug. What is the time required? dead cells should...

02 March 2016 4,498 0 View

Detecting active form of caspase 3 and 9 with western blotting?

Hello everyone, I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase...

01 February 2016 8,428 1 View

Treating cancer stem cells with drugs?

31 December 2015 9,346 1 View

Any advice on PI staining and FACS analysis on cancer stem cells?

dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...

11 December 2015 5,270 4 View

Migrating metatstatic cancer clusters?

Hello everyone, I would like to know the composition of migrating metastatic clusters generated by EMT. Any idea, please? Thanks in advance.

10 November 2015 1,670 2 View

How can I stain adherent cells with Annexin V ?

06 July 2015 2,564 2 View

Why am I experiencing high cytotoxicity when transfecting the T24 cell line?

06 July 2015 8,798 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View