Thomas Mühlenberg

6 Questions 89 Answers 0 Followers

Questions related from Thomas Mühlenberg

What's the second band in my cDNA after RT-PCR and RNAse H?

Hello, I am trying to generate ssDNA from an RNA template via RTPCR and consecutive digestion of the remaining/duplexed RNA by RNAse H. The first part is easy, but if I run my ssDNA on a gel I get...

11 November 2018 7,016 0 View

Using Triton X-114 to deplete endotoxin in my plasmid prep - when and how?

Hello, I am working with endotoxin sensitive cells now and electroporation is quite toxic to them compared to an endotoxin free control. So I think I am going to clean up my existing plasmids...

09 September 2016 8,225 6 View

Does anyone know a company to buy a used (and broken) Beckman Coulter FACS in Germany - or do you want it?

Hi, Our about 8 year old FACS, Beckmann Coulter FC500, has now a broken flow cell (literally) and we decided against a repair but to buy a new machine. Is anyone aware of a company (preferably...

02 February 2016 5,845 1 View

How can I linearize large amounts of plasmid?

I am facing a few problems linearizing my vector for electroporation: I want to linearize large amounts, say 50µg, of the vector (~14k bp), and have to use a rather expensive restriction enzyme...

07 July 2015 4,811 18 View

Can anyone tell me a more aggressive stripping method for western blots?

I was wondering, and didn't find so much on the web, if there was a stripping method for nitrocellulose membranes, to get rid of my previous stains. In our lab we use (I think the most common)...

01 January 2015 4,085 5 View

Is there a VEGFR2 dependent cell line?

I am looking for a (possibly cancer) cell line, that for proliferation depends on VEGFR2. Either a line that has activating mutations or depends on VEGF addition to the media. I want to use it as...

08 August 2014 3,072 2 View