PD-L1 detection after SDS-PAGE?

Hello, Does anyone have experience with PD-L1 detection by SDS-PAGE? I am using 2 antibodies from Cell signaling Technology and E-bioscience with apposite outcomes. Furthermore, when reprobing...

01 February 2020 8,658 3 View

How to remove EGF from cell culture medium?

Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...

11 December 2018 6,489 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

03 April 2016 4,209 4 View

Rhodamine 123 staining protocol for Flow cytometry?

03 April 2016 6,364 4 View

Rhodamine 123 staining?

Hello, I am planning to carry on a rhodamine 123 staining to measure the activity of multi drug resistant protein on cells after treatment with a drug. What is the time required? dead cells should...

02 March 2016 4,498 0 View

Detecting active form of caspase 3 and 9 with western blotting?

Hello everyone, I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase...

01 February 2016 8,428 1 View

Treating cancer stem cells with drugs?

31 December 2015 9,346 1 View

Any advice on PI staining and FACS analysis on cancer stem cells?

dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...

11 December 2015 5,270 4 View

How can I stain adherent cells with Annexin V ?

06 July 2015 2,564 2 View

Why am I experiencing high cytotoxicity when transfecting the T24 cell line?

06 July 2015 8,798 8 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View