Migrating metatstatic cancer clusters?

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PD-L1 detection after SDS-PAGE?

Hello, Does anyone have experience with PD-L1 detection by SDS-PAGE? I am using 2 antibodies from Cell signaling Technology and E-bioscience with apposite outcomes. Furthermore, when reprobing...

01 February 2020 8,809 3 View

How to remove EGF from cell culture medium?

Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...

11 December 2018 6,696 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

Hello, Is the permeabilization step essential in Rhodamine 123 mitochondria stainingin order to measure the mitochondrial potential? Do I need to dettach cells with Trypsin before...

03 April 2016 4,362 4 View

Rhodamine 123 staining protocol for Flow cytometry?

Hello, Could you share any reliable protocol for rhodamine 123 staining on adherent cells? Thanks in advance.

03 April 2016 6,553 4 View

Rhodamine 123 staining?

Hello, I am planning to carry on a rhodamine 123 staining to measure the activity of multi drug resistant protein on cells after treatment with a drug. What is the time required? dead cells should...

02 March 2016 4,694 0 View

Detecting active form of caspase 3 and 9 with western blotting?

Hello everyone, I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase...

01 February 2016 8,586 1 View

Treating cancer stem cells with drugs?

Hello everyone, I am planning to assess the effect of a drug on cancer stem cells proliferation. Giving that the mammosphere assay is an efficient way to assess cancer stem cells proliferation, I...

31 December 2015 9,520 1 View

Any advice on PI staining and FACS analysis on cancer stem cells?

dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...

11 December 2015 5,497 4 View

Why am I experiencing high cytotoxicity when transfecting the T24 cell line?

Hello everyone, Hello, I tried recently to transfect the T24 cell line using the Lipofectamine 3000 and the Amaxa nucleofection. Unfortunetly, 24 hours after the transfection 90% of cells were...

06 July 2015 8,986 8 View

How can I stain adherent cells with Annexin V ?

Hello everyone, I m looking for an Annexin V staining protocol for adherent cells without detaching cells from plate. As known, using trypsin to detach cells can increase considerably the number...

06 July 2015 2,738 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

15 July 2024 762 3 View

Oncologist at Visakhapatnam interested to collaborate?

Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...

14 July 2024 936 0 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View