15 Questions 95 Answers 0 Followers
Questions related from Hamadi Madhi
Hello, Does anyone have experience with PD-L1 detection by SDS-PAGE? I am using 2 antibodies from Cell signaling Technology and E-bioscience with apposite outcomes. Furthermore, when reprobing...
02 February 2020 8,756 3 View
Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...
12 December 2018 6,618 4 View
Hello, Could you share any reliable protocol for rhodamine 123 staining on adherent cells? Thanks in advance.
04 April 2016 6,496 4 View
Hello, Is the permeabilization step essential in Rhodamine 123 mitochondria stainingin order to measure the mitochondrial potential? Do I need to dettach cells with Trypsin before...
04 April 2016 4,310 4 View
Hello, I am planning to carry on a rhodamine 123 staining to measure the activity of multi drug resistant protein on cells after treatment with a drug. What is the time required? dead cells should...
03 March 2016 4,634 0 View
Hello everyone, I have a trouble in detecting the active form of caspase 3 and 9 using western blotting. Despite being successful in detecting the cleaved form of PARP, I failed to detect caspase...
02 February 2016 8,530 1 View
Hello everyone, I am planning to assess the effect of a drug on cancer stem cells proliferation. Giving that the mammosphere assay is an efficient way to assess cancer stem cells proliferation, I...
01 January 2016 9,440 1 View
dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...
12 December 2015 5,413 4 View
Hello everyone, I would like to know the composition of migrating metastatic clusters generated by EMT. Any idea, please? Thanks in advance.
11 November 2015 1,741 2 View
Hello everyone, Hello, I tried recently to transfect the T24 cell line using the Lipofectamine 3000 and the Amaxa nucleofection. Unfortunetly, 24 hours after the transfection 90% of cells were...
07 July 2015 8,910 8 View
Hello everyone, I m looking for an Annexin V staining protocol for adherent cells without detaching cells from plate. As known, using trypsin to detach cells can increase considerably the number...
07 July 2015 2,664 2 View
Hello everyone, I am working now on optimizing mammospheres culture condition for the MDA-MB231,MCF7 and 4T1 cell lines. My medium is a serum free medium DMEM/F12 supplemented with B27(2X),...
06 June 2015 9,208 3 View
Hello everyone, Is there any solution to reduce considerably the 4T1 mammospheres aggregation.Is there any other agent that can replace methyl cellulose?
05 May 2015 9,393 2 View
Hello everyone, I tried many times the T24 cell line using 2 different transfection agents(Lipofectamine 3000 and Xtreme Gene HP DNA) and using different concentrations of transfection reagents...
05 May 2015 664 3 View
Hello everyone, Anyone has an idea about the pH dissociation method to separate cells? I heard that this method is less harsh on the cells than traditional mechanical(pipetting) or enzymatic...
05 May 2015 9,003 4 View
