Hi,
I am seeing cell clumps in single cell suspension of splenocytes. I use DMEM media+HEPES+Gentamycin. Then I resuspend the cells in AIM-V media.
The cells get clumpy and they can even be seen by eyes.
What the possible solution might be?
Does anyone know whether I can add EDTA to splenic culture media during tissue processing and also 2-3 days of incubation ?