Hi,

I am seeing cell clumps in single cell suspension of splenocytes. I use DMEM media+HEPES+Gentamycin. Then I resuspend the cells in AIM-V media.

The cells get clumpy and they can even be seen by eyes.

What the possible solution might be?

Does anyone know whether I can add EDTA to splenic culture media during tissue processing and also 2-3 days of incubation ?

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