Hello,
I have a problem when revealing my western blots ! I use biorad LF PVDF membranes with the Biorad transfer system (in 7 min) I block with the Biorad blocking solution then I incubate with the primary anticorp and I reveal again with the Biorad SB 700 rabbit. Nevertheless, the membranes appear as they do here, and it's easy to understand why!
It's been a while since I changed all the solutions I usually use, but we still manage to get this profile!
has anyone encountered this problem before?
thanks for your help
Hello,
the resolution of your bands looks fine and your lanes as well.
For me, it looks like the problem is coming from the apparatus you use to take your image.
The image you show comes from an ECL revelation or fluorescent one ?
Hello,
Thank you for your reply, it's a fluorescent revelation and we use ChemiDoc Biorad to reveal. I don't think it's the apparatus because, it's reveal other membranes normally !
Mina Tizerarine ok!
is there a difference between the four lanes on the left and the four ones on the right ?
strange... I would say that in your 4 first lanes you have a very strong signal that leaks on the gel ... when the signal is very stong it can makes white bands instead of black ones... did you make a spot with a pencil ? (inks are fluorescent )...