How should I deal with missing data?

12 February 2021 5,306 4 View

No title

01 January 2021 9,970 2 View

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

16 November 2020 9,829 1 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

What is the best blocking agent to block rGO biosensors?

I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?

05 November 2020 5,140 3 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

29 October 2020 3,229 7 View

What kind of MMP should I use If I want to degrade rat tail collagen (Type I) gels?

I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...

28 July 2020 6,711 1 View

How can i test the Security for an algorithm in the field of IoT for a paper?

I need to know how to test the security of an implemented algorithm in the field of the IoT to state the test results in a paper. #IoT #Security #Blockchain #remix #Smart_Contract

01 July 2020 7,390 11 View

How to run Difference in Difference Different Time Periods?

Dear All, I have Panel Data fits difference in difference I regress the (Bilateral Investment Treaties-BIT) on (Bilateral FDI). BIT: is dummy taking 1 if BIT exists and Zero Otherwise....

29 May 2020 9,871 6 View

Thermodynamic properties of of the components of a certain solar thermophotovoltaic?

I am trying to simulate a solar TPV with a '1D Si/SiO2 PhC Emitter' and a single diode 'InGaAsSb pv cell'. now I am looking for 'thermal conductivity', 'Specific heat capacity' and 'density' of...

25 May 2020 7,818 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Pseudomonas that lose their fluorescence?

I am working on Pseudomonas fluorescent strain identification, I found that some of isolate that I have been conserve at 80c last year have loosing their fluorscent appearance on new culture....

09 November 2020 9,181 4 View

Can anyone tell me the list of antibiotics which are sensetive and resistanc to Pseudomonas flourences and Pseudomonas puituda bacteria ?

I am now working on conjugation process between Pseudomonas flourences and Pseudomonas puituda bacteria, so in order to confirm that the genes are transferred, we need to use antibiotics, so...

31 October 2020 509 3 View

How many ways do bacteria have to communicate?

Hello, I am researching into how bacteria communicate. What I've found is that they use quorum-sensing*, and electrical signals**. I've also come across the ability to "speak and listen"***, but I...

03 September 2020 9,168 8 View

The medium used in LasA staphylolytic assay and LasB Elastin congo red assay?

I'm currently collecting references about staphylolytic assay to detect LasA protease activity and elastin congo red assay to detect LasB elastase activity in Pseudomonas aeruginosa, but from the...

03 August 2020 1,160 3 View

Bacterial siderophore production test/modified CAS assay results interpretation?

I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...

26 July 2020 7,194 4 View

NirS qPCR assay underestimating by 2 log?

My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...

20 July 2020 8,070 1 View

Why are bacteria washed with NaCl solutions or some specific buffer before any photolysis experiment?

People who work with bacteria, I have been looking at literature and every time a photolysis experiment to thermally kill bacteria is done, the bacteria are spun down, the supernatant growth...

14 May 2020 9,071 5 View