Mina Zhiani

2 Questions 1 Answers 0 Followers

Questions related from Mina Zhiani

Is there any way to prevent murine splenocytes from clumping?

Hi, I am seeing cell clumps in single cell suspension of splenocytes. I use DMEM media+HEPES+Gentamycin. Then I resuspend the cells in AIM-V media. The cells get clumpy and they can even be seen...

01 August 2023 5,550 0 View

Why there are non-specific IL-5 secreting cells in the lungs of mice?

Hello all, I'm studying Th-1, Th-2 and Th-17 response in vaccinated and control mice groups. I perform T-cell cytokine Elispot (IFN-gamma, IL-5 and IL-17) on lung and spleen tissues. I've faced an...

01 August 2023 7,921 0 View