Hello
Cancer cells are known for their ability to grow quickly and many people culture with a reduced serum and the reduced serum keeps them growing and dividing . for that i suggest The best thing you can do is plate out some wells of your cells and place different wells under different serum percentages and check to see how this effect in growth . This way you can get to know explanation of change in the growth pattern and morphology in your cell line and learn how they respond to serum changes .
Also , find the artical in attachment " Serum-dependent Growth Patterns of Two, Newly Established Human Mesothelioma Cell Lines1", where refer Julius Klominek, et al in that the choice of serum can affect the in vitro morphology of the established mesothelioma cell lines .
Hope help you
Good Luck
http://www.ncbi.nlm.nih.gov/pubmed/7004631
You are right Gabriel. Mycoplasma cannot be detected by microscope yet their presence leads to drastic changes in morphology of cells.
Also reduced serum concentration leads to stress on the cells.
So you must check for the serum concentration specific for your cell line,normally we use 10%,which works well for almost all cell lines.
If it still affects morphology,I suggest you check for Mycoplasma contamination by PCR.
Yes, there could be changes depending on what sera you use .Better follow a standard procedure.
Hi Chandra,
You can observe changes in cell growth if you change serum batch.
Usually not morphology changes if you use same serum type with same concentration.
You can have morphology changes if you use other specie serum or concentration like Saleh said or another choice is the your cells are infected by mycoplasma like Gabriel said.
Virginie.
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