What are the good journals in cancer field which don't charge for publication??

I want to know about the journals which don't charge for publication of an article and also the one which takes less time in processing.

03 April 2017 6,880 4 View

What concentration of TGF beta and TNF alpha should i use in SW480 and SW620 cell lines for pathway induction

I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.

11 December 2016 8,019 2 View

How many times can we use the same ?

1- 3% BSA blocking ? 2- Primary antibody 3- HRP conjugated secondary antibody 4- Transfer buffer 5- Running buffer

04 May 2016 919 3 View

What are the different grades of water used in molecular biology work.How we determine which grade to be used in what experiment ?

Milli Q, single distilled, double distilled, autoclaved, deionized - these are the words i encounter before water.I confuse a lot to understand these words.Is there any way to quickly determine...

10 November 2015 171 1 View

How many passages of colon cancer and cervical cell line can be maintained in the same dish or flask continuously without affecting the assays?

After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.

09 October 2015 4,412 11 View

Is there any effect of change in serum on cancer cell line?

I am observing some change in growth pattern and morphology

09 October 2015 3,826 5 View

Why should we run SDS-PAGE on constant voltage and Western Blot transfer on constant current ?

11 December 2014 9,857 8 View

Does dmso of different grades make a difference in phytochemical drug solubility? Which is the best grade to solubilize phytochemical drug?

My drug is getting precipitated.

09 October 2014 10,022 6 View

Hesperidin is getting precipitated - How can I solve this problem?

I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...

08 September 2014 8,791 6 View

Can anyone tell me is it OK to use 0.6 % highest conc. of DMSO in MTT for SW 620 and SW 480 cell lines?

I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.

06 July 2014 6,127 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View