Chandra Kishore

23 Questions 8 Answers 0 Followers

Questions related from Chandra Kishore

What are the good journals in cancer field which don't charge for publication??

I want to know about the journals which don't charge for publication of an article and also the one which takes less time in processing.

04 April 2017 6,966 4 View

What concentration of TGF beta and TNF alpha should i use in SW480 and SW620 cell lines for pathway induction

I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.

12 December 2016 8,123 2 View

How many times can we use the same ?

1- 3% BSA blocking ? 2- Primary antibody 3- HRP conjugated secondary antibody 4- Transfer buffer 5- Running buffer

05 May 2016 1,001 3 View

What are the different grades of water used in molecular biology work.How we determine which grade to be used in what experiment ?

Milli Q, single distilled, double distilled, autoclaved, deionized - these are the words i encounter before water.I confuse a lot to understand these words.Is there any way to quickly determine...

11 November 2015 251 1 View

How many passages of colon cancer and cervical cell line can be maintained in the same dish or flask continuously without affecting the assays?

After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.

10 October 2015 4,485 11 View

Is there any effect of change in serum on cancer cell line?

I am observing some change in growth pattern and morphology

10 October 2015 3,897 5 View

Why should we run SDS-PAGE on constant voltage and Western Blot transfer on constant current ?

Protocols disagree on the exact voltage and current set up for the above technique

12 December 2014 10,161 8 View

Does dmso of different grades make a difference in phytochemical drug solubility? Which is the best grade to solubilize phytochemical drug?

My drug is getting precipitated.

10 October 2014 10,118 6 View

Hesperidin is getting precipitated - How can I solve this problem?

I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...

09 September 2014 8,869 6 View

Can anyone tell me is it OK to use 0.6 % highest conc. of DMSO in MTT for SW 620 and SW 480 cell lines?

I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.

07 July 2014 6,212 6 View

What maximum IC-50 conc. of phytochemical drug in MTT is acceptable for publication?

Is it ok if ic50 is 1mM for the phytochemical drug ?

07 July 2014 316 7 View

Which solvent is best for dissolving hesperidin for an MTT assay on a cancer cell line?

In DMSO solvent, the DMSO control is showing more effect than the drug.

06 June 2014 4,459 3 View

Which is more toxic to colon cancer cells - DMSO or ethanol?

How to inhibit ethanol evaporation if it is used as solvent for dissolving drug in MTT assay

06 June 2014 644 9 View

What is the meaning of 5p and 3p in micro RNA - miR140-5p/140-3p ?

Is it different from passenger and guide strand?

06 June 2014 6,756 5 View

What does modal number of a cell line mean?

For HCT 116, the modal number at 45 is 62 %. What does it mean ??

01 January 2014 2,800 3 View

Which cancer cell line shows maximum chromosomal stability?

I am planning to use lentiviral construct for some gene knockdown and study effect on chromosome.

01 January 2014 1,618 3 View

Is there any effect of 70% alcohol used widely for washing hand in lab

Does it penetrate skin or show any allergenic reaction.

12 December 2013 7,431 1 View

Does the micro RNA act as a epigenetic modifier?

Any example where micro RNA is involved in regulation, modification or interaction with DNMT, HM, HDACs or any other epigenetic regulator?

11 November 2013 4,832 7 View

Is there any example of down regulation of micro RNA gene transcription by its promoter CpG hypermethylation in cervical cancer cell lines?

Epigenetic down regulation of micro RNA gene has been discovered in many types of cancers.

11 November 2013 8,801 1 View

Will storing SiHa cancer cell line in a -80 degrees Celsius freezer (with 10 % DMSO in FBS) work?

Will this storage method be successful?Any suggested amendments?

11 November 2013 400 4 View

What are the mutation causing factors in BCR-ABL sequence, when the patient is treated for a long time with Gleevec?

Substitution of 13 amino acids were found to be present in kinase domain of BCR-ABL in Gleevec resistant tumour.

11 November 2013 2,187 2 View

Why, when cells are kept in closed t-flask and CO2 flow is in incubator, that the CO2 is able to maintain PH if it doesn't enter the t-flask?

In tissue culture.

10 October 2013 10,016 3 View

What is the difference between siRNA and micro RNA?

The process of formation is almost the same for the both of them. Why exactly are they named differently?

10 October 2013 4,207 3 View