What concentration of TGF beta and TNF alpha should i use in SW480 and SW620 cell lines for pathway induction

I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.

11 December 2016 8,019 2 View

How many times can we use the same ?

1- 3% BSA blocking ? 2- Primary antibody 3- HRP conjugated secondary antibody 4- Transfer buffer 5- Running buffer

04 May 2016 919 3 View

What are the different grades of water used in molecular biology work.How we determine which grade to be used in what experiment ?

Milli Q, single distilled, double distilled, autoclaved, deionized - these are the words i encounter before water.I confuse a lot to understand these words.Is there any way to quickly determine...

10 November 2015 171 1 View

How many passages of colon cancer and cervical cell line can be maintained in the same dish or flask continuously without affecting the assays?

After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.

09 October 2015 4,412 11 View

Is there any effect of change in serum on cancer cell line?

I am observing some change in growth pattern and morphology

09 October 2015 3,826 5 View

Why should we run SDS-PAGE on constant voltage and Western Blot transfer on constant current ?

11 December 2014 9,857 8 View

Does dmso of different grades make a difference in phytochemical drug solubility? Which is the best grade to solubilize phytochemical drug?

My drug is getting precipitated.

09 October 2014 10,022 6 View

Hesperidin is getting precipitated - How can I solve this problem?

I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...

08 September 2014 8,791 6 View

Can anyone tell me is it OK to use 0.6 % highest conc. of DMSO in MTT for SW 620 and SW 480 cell lines?

I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.

06 July 2014 6,127 6 View

What maximum IC-50 conc. of phytochemical drug in MTT is acceptable for publication?

Is it ok if ic50 is 1mM for the phytochemical drug ?

06 July 2014 224 7 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View