One addition to Steingrimor: You can't orient it "any way you want" if your cells depend on specific CO2/O2 conditions or such. Basically the environment in a deep liquid volume is different from a flat liquid volume (no shaking means most O2 is in the upper volume, little on the bottom). A vertical position means you have a clear O2/CO2 gradient through larger volumes. In a horizontal setup the cells are usually in a pretty thin volume layer, so the gas exchange is easier and more equal.
So it depends on the conditions for gas exchange you want. For normal suspension cell lines though you can usually easily go for horizontal setups.
no; u can even keep your suspension culture horizontally (since many stands it vertica)
As long as you don't block the vented caps you can orient the flasks any way you want.
One addition to Steingrimor: You can't orient it "any way you want" if your cells depend on specific CO2/O2 conditions or such. Basically the environment in a deep liquid volume is different from a flat liquid volume (no shaking means most O2 is in the upper volume, little on the bottom). A vertical position means you have a clear O2/CO2 gradient through larger volumes. In a horizontal setup the cells are usually in a pretty thin volume layer, so the gas exchange is easier and more equal.
So it depends on the conditions for gas exchange you want. For normal suspension cell lines though you can usually easily go for horizontal setups.
Its not a problem how to kept the flask, only thing you should care about aseptic condition of cell culture
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