Can anyone suggest to me the tool for gene annotation and clustering of microarray data?

31 December 2014 5,551 3 View

Does anyone (in India) have Jurkat E6.1 and SH-SY5Y cell lines?

As NCCS Pune has stopped to supply these cell lines, I am not able to get them.

01 February 2014 2,841 2 View

Microarray raw data submission?

What is the procedure to submit raw data of microarrays? In which microarray database I should submit my raw data and is it compulsory to submit the data before communicate the article?

31 December 2013 2,307 6 View

Anyone suggest me how to lyophilize the 2D-Protein gel plugs for the MALDI-TOF analysis?

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11 December 2013 9,854 1 View

Can anyone suggest a suitable cell line for a study of the effects of neurotoxic agents on human neuroblastoma?

We have an IMR-32 cell line. Can anyone suggest whether this is a good cell line for the above study?

09 October 2013 9,083 3 View

How to do mitochondrial membrane potential?

I want to do mitochondrial membrane potential of human cell line. can anyone tell me the procedure of the assay? *We don't have flowcytometer in our lab.

08 September 2013 5,027 7 View

Hoechst staining of HL-60 cell line?

I would like to perform fluorescence microscopy on a HL-60 cell line using Hoechst. How to stain the suspension culture with this stain? Can anybody send me a detailed protocol?

08 September 2013 1,707 2 View

How to lyse cell culture for the analysis of ROS/RNS using kit ??

I am planing to use a kit [(from cell biolabs (cat # STA-347) ] for the analysis of total ROS. In this kit it is given to sonicate or homogenize the cells but we don't have sonicator in our lab....

05 June 2013 1,933 4 View

Micronucleus assay on HL-60 cell line?

I am doing this assay on HL-60 cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei. Does anyone have experience with this assay? Please send me any...

05 June 2013 9,850 3 View

Fungal contamination in cell culture flask.

I have observed fungal contamination in my used empty culture flask (after trypisinization) when incubated in CO2 incubator (dedicated for cell culture). However I haven't seen any problems in my...

04 May 2013 735 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View