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Questions related from Ananda Ayyappan Jaguva Vasudevan
While I found a prominent 58kDa (others 37.4, and 27.3 kDa forms) from data banks, Domanski et al 1995 JBC presented a 110 kDa isoform. I am not sure about this really. Please share your expertise...
02 February 2018 6,594 2 View
If I have an antibody enriched in a sample and I would like to know which antigen to employ, what techniques can I use? Do you think methods such as co-IP coupled with mass spec analysis is the...
01 January 2013 912 18 View
I would like to check the expression profile of some proteins of PBMC and PBMC derived macrophages(with treatments). For the same purpose, what would be a good HKG for western blot? Any of your...
12 December 2012 2,664 3 View
I have a fusion protein separated by thrombin cleavage site with GST. I eluted my fusion protein with 20mM Glutathione(reduced),50mM Tris(pH8),500mM NaCl,10mM CaCl2,10% glycerol and 5mM 2-ME. This...
12 December 2012 7,627 16 View
To avoid the carry over of plasmids with virus genome used in transfection, we treat the virus supernatant with DNAse I. In what way I can increase the efficiency of the reaction? Is the DNAse I...
11 November 2012 1,539 21 View
Why do some protocol uses CaCl2 in the lysis and elution buffers while purifying the soluble protein from E.coli?
11 November 2012 4,231 2 View
I would like to make primers for my qPCR. For that, I need a reliable software. I know the (ABI) Primer express software(paid) is preferred, but do you know any freeware that does the job well?
09 September 2012 7,900 12 View
Why do we need BSA as a blocking buffer for certain antibodies? What is different from casein? Thanks, Anand
06 June 2012 550 4 View
Hi, does any one has experience with Subtilisin A for the digestion of Viral core/membrane protein or any other? please share your knowledge.
01 January 2012 3,715 11 View
I have some problem with my protein purified via IMAC(native condition), after my IMAC purification, when I try to concentrate eluates, considerable amount of protein start aggregates! I tried...
12 December 2011 3,267 7 View
