Ulrike Jung

5 Questions 70 Answers 0 Followers

Questions related from Ulrike Jung

Can anyone recommend chromatography machines for vector purification?

Hi, we are in need of chromatography systems and looking at what is available at the market. Needless to say I haven't used this technique yet so starting from scratch. We need to purify viral...

01 January 2016 6,459 3 View

Which large scale Tangential Flow purification system is recommended for vector purification?

Is anyone out here who has done vector purification with TFF? I'm looking to compare experiences with systems, especially anything above 1 liter. What did you use, what were your experiences,...

11 November 2015 8,476 1 View

Q/RT-PCR machine, what company/model would you prefer?

We are looking to buy a new machine and are looking at any available option. This is not for extremely special applications but regular RT-PCR, but we are interested in a device which is extremely...

11 November 2015 3,418 4 View

What is the optimum HEK 293T cell seeding cells/cm2 for large scale culture and lentiviral production?

After over a decade of seeding small(er) scale 293T fresh from ATCC I can say with confidence that Invitrogen (HeLa based) chart never really worked out for me to obtain 70-80% for 293T, the...

01 January 2015 6,569 16 View

Does anyone have data about the effect of removing the intron from hEF1a?

I'm trying to figure out if someone has just removed the intron and checked what the effect on expression is (preferentially in genomically integrated cassettes) when you use the short/core EF1a...

11 November 2013 4,236 1 View