25 Questions 29 Answers 0 Followers
Questions related from Deepa Gandhi
I am trying to do the analysis with DAVID but their is some problem with the software. Please suggest software which is freely available.
01 January 2015 5,615 3 View
I used PI stain for Jurkat cell line, in this control (normal) cells also give fluorescence. Can anyone suggest what can be the problem?
04 April 2014 3,594 3 View
As NCCS Pune has stopped to supply these cell lines, I am not able to get them.
02 February 2014 2,902 2 View
What is the procedure to submit raw data of microarrays? In which microarray database I should submit my raw data and is it compulsory to submit the data before communicate the article?
01 January 2014 2,379 6 View
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12 December 2013 9,913 1 View
We have an IMR-32 cell line. Can anyone suggest whether this is a good cell line for the above study?
10 October 2013 9,142 3 View
I would like to perform fluorescence microscopy on a HL-60 cell line using Hoechst. How to stain the suspension culture with this stain? Can anybody send me a detailed protocol?
09 September 2013 1,780 2 View
I want to do mitochondrial membrane potential of human cell line. can anyone tell me the procedure of the assay? *We don't have flowcytometer in our lab.
09 September 2013 5,102 7 View
I am planing to use a kit [(from cell biolabs (cat # STA-347) ] for the analysis of total ROS. In this kit it is given to sonicate or homogenize the cells but we don't have sonicator in our lab....
06 June 2013 1,995 4 View
I am doing this assay on HL-60 cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei. Does anyone have experience with this assay? Please send me any...
06 June 2013 9,910 3 View
I have observed fungal contamination in my used empty culture flask (after trypisinization) when incubated in CO2 incubator (dedicated for cell culture). However I haven't seen any problems in my...
05 May 2013 817 12 View
04 April 2013 4,801 5 View
I am working with L6 cell line and it is continuously forming clumps. Can anyone suggest how I can avoid clump formation?
04 April 2013 9,364 2 View
Why do we calculate only LC50? Why not 30, 40 or other values?
04 April 2013 244 16 View
I don't know, in my study model (human cell line), which transcript variant is going to be expressed.
04 April 2013 6,446 10 View
I want to do gene expression analysis of cell lines exposed to pesticides with control cells using microarray. Which chip should I select for this study?
04 April 2013 8,868 3 View
Please suggest a stabilized protocol..
03 March 2013 6,802 5 View
I am using silver nanoparticles, 20 wt. % (dispersion in organic solvents) (sigma, product # 719048) for an MTT assay on a HL-60 cell line and I am not getting what should be my vehicle control?
02 February 2013 1,641 1 View
I have isolated DNA from a human cell line using the Phenol-Chloroform method and when I run the gel I see very bright bands (3 bands of rRNA) of RNA with DNA. The phenol which I used was fresh...
02 February 2013 3,560 20 View
I want sterile 1 N HCl, can autoclave it?
02 February 2013 5,377 19 View
What will happen if i use DMEM or MEM media for these cultures (e.g. Jurkat, HL-60 etc.) ?
02 February 2013 2,997 1 View
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02 February 2013 9,638 9 View
How can I isolate RNA from cell line suspended in RNAlater (I mean, how can I remove the RNAlater)? I use TRIzol for RNA isolation, does RNA later interfere with downstream processing (i.e. real...
02 February 2013 2,494 7 View
In any suspension culture where all cells have similar density, why do the cells float in different layers inside the media? Is it necessary to keep a flask with these cultures in a vertical...
02 February 2013 5,186 3 View
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02 February 2013 1,350 13 View
