Can anyone suggest to me the tool for gene annotation and clustering of microarray data?

31 December 2014 5,551 3 View

Can anyone help me with PI staining of the cell line?

I used PI stain for Jurkat cell line, in this control (normal) cells also give fluorescence. Can anyone suggest what can be the problem?

03 April 2014 3,521 3 View

Does anyone (in India) have Jurkat E6.1 and SH-SY5Y cell lines?

As NCCS Pune has stopped to supply these cell lines, I am not able to get them.

01 February 2014 2,841 2 View

Microarray raw data submission?

What is the procedure to submit raw data of microarrays? In which microarray database I should submit my raw data and is it compulsory to submit the data before communicate the article?

31 December 2013 2,307 6 View

Anyone suggest me how to lyophilize the 2D-Protein gel plugs for the MALDI-TOF analysis?

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11 December 2013 9,854 1 View

Can anyone suggest a suitable cell line for a study of the effects of neurotoxic agents on human neuroblastoma?

We have an IMR-32 cell line. Can anyone suggest whether this is a good cell line for the above study?

09 October 2013 9,083 3 View

How to do mitochondrial membrane potential?

I want to do mitochondrial membrane potential of human cell line. can anyone tell me the procedure of the assay? *We don't have flowcytometer in our lab.

08 September 2013 5,027 7 View

How to lyse cell culture for the analysis of ROS/RNS using kit ??

I am planing to use a kit [(from cell biolabs (cat # STA-347) ] for the analysis of total ROS. In this kit it is given to sonicate or homogenize the cells but we don't have sonicator in our lab....

05 June 2013 1,933 4 View

Micronucleus assay on HL-60 cell line?

I am doing this assay on HL-60 cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei. Does anyone have experience with this assay? Please send me any...

05 June 2013 9,850 3 View

Fungal contamination in cell culture flask.

I have observed fungal contamination in my used empty culture flask (after trypisinization) when incubated in CO2 incubator (dedicated for cell culture). However I haven't seen any problems in my...

04 May 2013 735 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View