I am doing this assay on HL-60 cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei. Does anyone have experience with this assay? Please send me any optimized protocol for this assay.
HL-60 cells are very sensitive, and are easily damaged. Plz refer to the following article, hope it will help....http://www.icb.ufmg.br/biq/prodap/2003/micronucleos/mn1.pdf