I am new to western blot, i want to check nfkappa-b p65 protein in cell, i ran western blot, i have some doubts, regarding my results?

I am checking effect of my compound on breast cancer cells, i ran western blot, i am attaching photo, and i have following questions- 1- i have not used phosphatase inhibitor in sample preparation...

01 February 2020 6,549 3 View

I got diffused bands in western blott? What could be the reason?

I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-

01 February 2020 3,050 2 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

09 October 2019 1,291 5 View

How to distinguish cell debris and contamination?

I am culturing breast cancer cell line, some time I observe high density of tiny dot like structure, I am not able to find out whether its contamination or cell debris.if it is contamination then...

01 February 2019 6,957 1 View

I am facing problem in maintaining breast cancer cell lines?

I am beginner in mammalian cell culture, I am maintaining breast cancer cell line (t47d,mdamb231,mdamb468,mcf7). Cells were going well but suddenly i observed so much cell debris and slightly...

01 February 2019 9,794 4 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

11 December 2018 9,689 5 View

How to dissolve arsenic trioxide loaded plga nanoparticles ?

I have prepared arsenic trioxide loaded nanoparticles...now i have to calculate Ic50 for the same, can anybody suggest me which solvent would be best to dissolve these...

10 November 2018 5,868 1 View

I have to calculate encapsulation efficiency of Arsenic loaded PLGA nanoparticles?

07 August 2018 6,297 7 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

05 June 2018 9,502 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View