I am new to western blot, i want to check nfkappa-b p65 protein in cell, i ran western blot, i have some doubts, regarding my results?

I am checking effect of my compound on breast cancer cells, i ran western blot, i am attaching photo, and i have following questions- 1- i have not used phosphatase inhibitor in sample preparation...

01 February 2020 6,549 3 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

09 October 2019 1,291 5 View

How to distinguish cell debris and contamination?

I am culturing breast cancer cell line, some time I observe high density of tiny dot like structure, I am not able to find out whether its contamination or cell debris.if it is contamination then...

01 February 2019 6,957 1 View

I am facing problem in maintaining breast cancer cell lines?

I am beginner in mammalian cell culture, I am maintaining breast cancer cell line (t47d,mdamb231,mdamb468,mcf7). Cells were going well but suddenly i observed so much cell debris and slightly...

01 February 2019 9,794 4 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

11 December 2018 9,689 5 View

How to dissolve arsenic trioxide loaded plga nanoparticles ?

I have prepared arsenic trioxide loaded nanoparticles...now i have to calculate Ic50 for the same, can anybody suggest me which solvent would be best to dissolve these...

10 November 2018 5,868 1 View

I have to calculate encapsulation efficiency of Arsenic loaded PLGA nanoparticles?

07 August 2018 6,297 7 View

I am running sds page first time..without ot protein marker..i have taken 10 ulit (2mg/ml) of bsa protein + 2x loading dye?

After running gel for 2 and half hour at 90v , n staining with cumassie i got more then one band...what might be reason ? I am attaching pic.

07 August 2018 472 5 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

05 June 2018 9,502 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View